Integrated Plant Genetics Inc.
6911 NW 22nd St., Ste C
Gainesville, FL 32653

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Adding Value to Crops and Foods with Advanced Gene & GRAS Technologies

Tel: +1 (386) 418-3494
Fax: +1 (352) 338-7599

Integrated Plant Genetics

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Citrus Canker In-Depth

I. Economic Hosts

II. Pathogens

III. Disease

IV. Symptoms and Signs

V. Disease

VI. Identification

VII. Pathogenicity

VIII. Storage of Organism:

Strains may be stored by lyophilization, by freezing, with silica gel or in sterile tap water. For freezer storage, media containing 15% glycerol is suitable, and strains may be held at -80 C or in liquid nitrogen. In silica gel storage, bacteria are suspended in 0.5 ml of 10% aqueous dry milk powder and mixed with 3 g sterilized anhydrous silica gel (Davison Chemical Co. Commercial grade 40, type 2, 6-12 mesh) in chilled storage tubes [17].

A very convenient method is storage in sterile tap water. Tap water containing high levels of calcium is most appropriate; deionized or distilled water is not suitable. Several loopfuls of bacteria may be scraped off a freshly streaked agar plate, suspended in 2 mls of sterile tap water, and stored at room temperature for many years in screw capped vials with a teflon seal. Strains die within six weeks on all agar media tested, whether refrigerated or not.

IX. Reported Host Range

X. Geographical Range and Spread

XI. Suggested Taxonomic Keys

XII. References

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Copyright © October 2001 Integrated Plant Genetics, Inc. -- All Rights Reserved

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