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Citrus Canker In-Depth
I. Economic Hosts
II. Pathogens
III. Disease
IV. Symptoms and Signs
V. Disease
VI. Identification
VII. Pathogenicity
VIII. Storage of Organism:
Strains may be stored by lyophilization, by freezing, with silica gel or in sterile
tap water. For freezer storage, media containing 15% glycerol is suitable, and
strains may be held at -80 C or in liquid nitrogen. In silica gel storage, bacteria
are suspended in 0.5 ml of 10% aqueous dry milk powder and mixed with 3 g
sterilized anhydrous silica gel (Davison Chemical Co. Commercial grade 40,
type 2, 6-12 mesh) in chilled storage tubes [17].
A very convenient method is
storage in sterile tap water. Tap water containing high levels of calcium is most
appropriate; deionized or distilled water is not suitable. Several loopfuls of
bacteria may be scraped off a freshly streaked agar plate, suspended in 2 mls of
sterile tap water, and stored at room temperature for many years in screw capped
vials with a teflon seal. Strains die within six weeks on all agar media tested,
whether refrigerated or not.
IX. Reported Host Range
X. Geographical Range and Spread
XI. Suggested Taxonomic Keys
XII. References
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Copyright © October 2001 Integrated Plant Genetics, Inc. -- All Rights Reserved
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