Integrated Plant Genetics Inc.
6911 NW 22nd St., Ste C
Gainesville, FL 32653

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Adding Value to Crops and Foods with Advanced Gene & GRAS Technologies

Tel: +1 (386) 418-3494
Fax: +1 (352) 338-7599

Integrated Plant Genetics

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Citrus Canker In-Depth

I. Economic Hosts

II. Pathogens

III. Disease

IV. Symptoms and Signs

V. Isolation:

X. citri strains may be isolated and cultured from all affected plant tissues by commonly used methods. Lesions are excised with a scalpel or razor, washed with tap water, surface sterilized for 3 minutes in a 10% dilution of commercial hypochlorite bleach, rinsed and sectioned. The water-soaked tissue at the lesion margin is dragged across a sterile agar medium containing 50 µg/ml kasugamycin. X. citri strains grow well on various nutrient agar media, and the following works well: 0.5% tryptone, 0.3% yeast extract, 0.09% CaCl2, 0.05% K2HPO4 and 1.5% agar in tap water, pH 7.2. We typically buffer this medium with 100mM morpholinopropane sulfate (MOPS) plus micronutrients (TY-MOPS) [7]. Yellow mucoid colonies should appear within 48 hours.

X. campestris pv. aurantifolii strains are reportedly difficult to isolate and culture directly from citrus tissue; these strains may be cultured initially on 1% sucrose, 0.5% peptone, 0.05% K2HPO4, 0.03% MgSO4 and Difco purified agar [2]. After initial culturing, however, these strains appear to adapt to other media and may be routinely cultured on TY-MOPS or other nutrient media.

VI. Identification

VII. Pathogenicity

VIII. Storage of Organism

IX. Reported Host Range

X. Geographical Range and Spread

XI. Suggested Taxonomic Keys

XII. References

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