Citrus Canker In-Depth
I. Economic Hosts
IV. Symptoms and Signs
X. citri strains may be isolated and cultured from all affected plant tissues
by commonly used methods. Lesions are excised with a scalpel or razor, washed
with tap water, surface sterilized for 3 minutes in a 10% dilution of commercial
hypochlorite bleach, rinsed and sectioned. The water-soaked tissue at the lesion
margin is dragged across a sterile agar medium containing 50 µg/ml kasugamycin.
X. citri strains grow well on various nutrient agar media, and the following
works well: 0.5% tryptone, 0.3% yeast extract, 0.09% CaCl2, 0.05% K2HPO4 and
1.5% agar in tap water, pH 7.2. We typically buffer this medium with 100mM
morpholinopropane sulfate (MOPS) plus micronutrients (TY-MOPS) . Yellow
mucoid colonies should appear within 48 hours.
X. campestris pv. aurantifolii strains are reportedly difficult to isolate and
culture directly from citrus tissue; these strains may be cultured initially on
1% sucrose, 0.5% peptone, 0.05% K2HPO4, 0.03% MgSO4 and Difco purified agar .
After initial culturing, however, these strains appear to adapt to other media
and may be routinely cultured on TY-MOPS or other nutrient media.
VIII. Storage of Organism
IX. Reported Host Range
X. Geographical Range and Spread
XI. Suggested Taxonomic Keys
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